The Department of Molecular Endocrinology has concentrated its efforts towards unraveling the complex pathophysiology of polycystic ovary syndrome (PCOS). This multifactorial syndrome manifests as a result of interaction between multiple genes and environmental factors. It is the leading cause of anovulatory infertility and is characterized by insulin resistance, androgen excess and polycystic ovaries with adverse long term reproductive and metabolic consequences. We have adopted several approaches to investigating its underlying pathogenic mechanism of this multifaceted syndrome.
Using candidate gene association studies, we have demonstrated association of genes involved in insulin resistance, oxidative stress and steroidogenesis with PCOS susceptibility and its related traits. Our studies have revealed that obesity may also influence the genetic pathogenesis of PCOS. We are currently investigating additional relevant genes in order to develop a genetic predisposition profile for Indian women with PCOS.
Environmental insults or in utero factors may induce chemical alterations in the chromatin (DNA methylation or histone modifications) by molecular epigenetic machinery, thus leading to altered gene expression.The epigenetic alterations associated with PCOS are also under investigation.
Our group has been instrumental in generating the largest protein dataset of follicular fluid till date. Furthermore, comparison of follicular fluid proteomes derived from normal and PCOS ovaries has revealed dysregulation of several biological processes vital for follicle development and maturation. The functional importance of several dysregulated proteins identified in PCOS is under investigation.
Angiogenesis is vital for the processes of healthy folliculogenesis as well as formation and functioning of corpus luteum, both of which are compromised in women with PCOS. We are currently working towards understanding the molecular mechanisms regulating angiogenesis in PCOS.
Fig.1: Proposed mechanism for arrest in follicular growth in women with PCOS. (Ambekaret al., 2015, JCEM)
Department Email Id: firstname.lastname@example.org
|Dr Srabani Mukherjee||Scientist ‘E’|
|Dr Pallavi Shukla||Scientist ‘C’|
|Mrs. Sushma Khavale||Technical Assistant|
|Mrs. Nanda Joshi||Technician ‘C’|
|Mr. Pradeep More||Technician ‘C’|
|Mrs. Gayatri Shinde||Technician ‘C’|
|Ms. Roshan Dadachanji||Ph D Scholar|
|Ms. Pooja Sagvekar||Ph D Scholar|
|Ms. Krutika Patil||Ph D Scholar|
- Understanding follicular angiogenesis in women with polycystic ovary syndrome
- Deciphering the Putative Epigenetic Mechanisms Pertaining to Polycystic Ovary Syndrome (funded by DST, Govt. of India, 2016-2019)
- PON1 expression, activity and its relationship with oocyte and embryo quality in women with PCOS undergoing assisted reproductive technique (Funded by BRNS, 2014-2017)
- Study of PON1gene variants & its association with cardiovascular disease risk factors in PCOS (Funded by DST, 2011-2014), presently intra-mural
- A genetic analysis of polycystic ovary syndrome with special emphasis on genes involved in insulin resistance (Funded by DST)
- The study of anti-apoptotic and pro-apoptotic molecules in the development of cystic follicles using an estrogen treated mouse model (Funded by BRNS)
- Human follicular fluid proteome in PCOS (Funded by DBT)