Confocal Laser Scanning Microscope

Confocal microscopy offers several advantages over conventional optical microscopy, including controllable depth of field, the elimination of image degrading out-of-focus information, and the ability to collect serial optical sections from thick specimens.

The Institute has a Confocal Laser Scanning Microscope (LSM) with spectral imaging. The system is mounted on an inverted microscope with temperature controlled stage. The laser systems include the Argon and Helium-neon lasers for fluorescence excitation of 458/488, 477, 514 and 633 nm, the DPSS and blue diode laser for 561, 405 nm excitation wavelengths. The scanning module has two single-channel detectors and polychromatic multichannel detector capable of simultaneous acquisition of up to 8 channels. Beside the feature of high resolution optical slicing and 3 dimensional imaging of the cells and tissues, it can also perform FRAP, FRET, FLIM and live cell imaging studies.

Investigators perform the labeling/staining as per requirements using appropriate flourophores in their laboratory. The system is available for viewing and analysis. Presently we have the expertise in co-localization and 3D reconstruction of the samples. Future plans are to include FRET and FRAP analysis as well.

Contact Person: Dr. Nafisa Balasinor: 91-22-24192025